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410 trnc 010 bmm stimulation  (R&D Systems)


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    R&D Systems 410 trnc 010 bmm stimulation
    410 Trnc 010 Bmm Stimulation, supplied by R&D Systems, used in various techniques. Bioz Stars score: 90/100, based on 37 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/410 trnc 010 bmm stimulation/product/R&D Systems
    Average 90 stars, based on 37 article reviews
    410 trnc 010 bmm stimulation - by Bioz Stars, 2026-03
    90/100 stars

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    ( A ) BMMs were left untreated or treated with TNF-α for 24 hr. Total RNA was used for RT-qPCR. Averages of technical duplicates for one biological replicate are shown. Data is representative of two independent experiments. ( B ) RT-qPCR of Sp140 –/– BMMs transduced with either control SINV-minCMV-GAL4-mNeonGreen (SINV-mNeonGreen) or SINV-minCMV-Sp140 (SINV-Sp140), primed with 5 ng/mL IFN-𝛾 for 14 hr and treated with 10 ng/mL TNF-⍺ for 4 hr. *p≤0.05 calculated with an unpaired t -test with Welch’s correction. Data are representative of two independent experiments. BMM, bone marrow-derived macrophage; RT-qPCR, real-time quantitative-polymerase chain reaction.
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    ( A ) BMMs were left untreated or treated with TNF-α for 24 hr. Total RNA was used for RT-qPCR. Averages of technical duplicates for one biological replicate are shown. Data is representative of two independent experiments. ( B ) RT-qPCR of Sp140 –/– BMMs transduced with either control SINV-minCMV-GAL4-mNeonGreen (SINV-mNeonGreen) or SINV-minCMV-Sp140 (SINV-Sp140), primed with 5 ng/mL IFN-𝛾 for 14 hr and treated with 10 ng/mL TNF-⍺ for 4 hr. *p≤0.05 calculated with an unpaired t -test with Welch’s correction. Data are representative of two independent experiments. BMM, bone marrow-derived macrophage; RT-qPCR, real-time quantitative-polymerase chain reaction.

    Journal: eLife

    Article Title: Role of the transcriptional regulator SP140 in resistance to bacterial infections via repression of type I interferons

    doi: 10.7554/eLife.67290

    Figure Lengend Snippet: ( A ) BMMs were left untreated or treated with TNF-α for 24 hr. Total RNA was used for RT-qPCR. Averages of technical duplicates for one biological replicate are shown. Data is representative of two independent experiments. ( B ) RT-qPCR of Sp140 –/– BMMs transduced with either control SINV-minCMV-GAL4-mNeonGreen (SINV-mNeonGreen) or SINV-minCMV-Sp140 (SINV-Sp140), primed with 5 ng/mL IFN-𝛾 for 14 hr and treated with 10 ng/mL TNF-⍺ for 4 hr. *p≤0.05 calculated with an unpaired t -test with Welch’s correction. Data are representative of two independent experiments. BMM, bone marrow-derived macrophage; RT-qPCR, real-time quantitative-polymerase chain reaction.

    Article Snippet: Cells were treated with 10 ng/ml recombinant mouse TNFα (410-TRNC-010, R&D Systems) diluted in the media as described above.

    Techniques: Quantitative RT-PCR, Transduction, Control, Derivative Assay, Real-time Polymerase Chain Reaction

    Journal: eLife

    Article Title: Role of the transcriptional regulator SP140 in resistance to bacterial infections via repression of type I interferons

    doi: 10.7554/eLife.67290

    Figure Lengend Snippet:

    Article Snippet: Cells were treated with 10 ng/ml recombinant mouse TNFα (410-TRNC-010, R&D Systems) diluted in the media as described above.

    Techniques: Cell Culture, Control, Injection, Recombinant, Plasmid Preparation, Derivative Assay, Sequencing